Summary updated: February 13, 2026 Estimated read: 4 min Primary source: Nature (2025)
Takarkori rock shelter in southwestern Libya where ancient DNA remains were recovered
Takarkori rock shelter, southwestern Libya. Image used by Nature coverage of Salem et al. (2025).
Ancient DNA Nature January 2025

Ancient DNA from the Green Sahara Reveals Ancestral North African Lineage

Salem, T. et al. (2025). Nature, 641, 144–150.

Summary

This landmark study recovered ancient DNA from human remains in the Takarkori rock shelter in southwestern Libya, dating to approximately 7,000 years ago during the "Green Sahara" period (African Humid Period). The Sahara was then a lush, habitable landscape supporting pastoralist communities.

The researchers successfully sequenced the genome of these ancient Saharan inhabitants and discovered they carried a previously unknown basal lineage of mitochondrial haplogroup N. This lineage diverged early from other Eurasian branches and had not been detected in any modern or ancient population previously studied.

The findings indicate that the Green Sahara harbored genetically distinct populations that contributed to the ancestry of later North African groups, but in complex ways that cannot be explained by simple models of north-south migration. The genetic isolation of these Saharan groups during the humid period suggests regional population structuring across North Africa during the Holocene.

Takarkori Site Location

Key Takeaways

Relevance to Our Lineage

While this study focuses on populations from a different time and place than the Chtouka lineage, it provides critical context for understanding the deep ancestry of all North African groups, including the Amazigh (Berber) populations of the Souss-Massa.

The discovery of a basal haplogroup N lineage in the Sahara demonstrates that North Africa's genetic landscape was shaped by multiple distinct population layers over millennia. This complexity supports the dual-origin model discussed on our Lineage History page, where indigenous North African paternal lineages (E-PF2546) coexist with maternal lineages (H1-T16189C!) that arrived via different migration routes.

The Green Sahara period represents one of several major demographic events that shaped the genetic diversity observed in modern Amazigh populations, preceding the later expansions that gave rise to the E-M81/E-PF2546 lineage.

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Lineage History Genetics